Archive

Expression of five different genes in transcript level was evaluated feeding with the supernatant of three symbiotic bacteria, Photorhabdus temperata, P. luminescence and Xenorhabdous nematophilus. Quantitative real-time PCR analysis revealed that the three bacterial media, individually or in combination, inhibited significantly the transcript levels of the almost all tested defense and cytoskeleton related genes, knottin 3, ferritin 2, ferritin 17, P8 protein and myosin H chain. The results suggest that cell free culture media of symbionts of entomopathogenic nematodes are highly lethal due to subdued expression of these defense and cytoskeleton genes.