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Insecticidal activities of culture media of three symbiotic bacteria, Photorhabdus temperata, P. luminescens and Xenorhabdus nematophilus, of entomopathogenic nematodes were tested against Bemisia tabaci. Adult B. tabaci showed significant mortality when rearing on the leaves which were dipped into 10% culture meidia of symbiotic bacteria. Complete mortality was observed at 60 h in 10% media of P. temperata and X. nematophilus, but mortality was less than 50% at 72 h in 10% P. luminescens. Otherwise, complete mortality was observed in 50% supernatants of three culture media with artificial diet within 60-66 h. In addition, complete mortality was observed in mixtures of 2 or 3 bacterial media of Pt+Pl, Pt+Xn, Pl+Xn, Pt+Pl+Xn within 54, 48, 48 and 42 h, respectively.